Increased expression of the autocrine motility factor is associated with poor prognosis in patients with clear cell-renal cell carcinoma
Articolo
Data di Pubblicazione:
2015
Citazione:
Increased expression of the autocrine motility factor is associated with poor prognosis in patients with clear cell-renal cell carcinoma / G. Lucarelli, M. Rutigliano, F. Sanguedolce, V. Galleggiante, A. Giglio, S. Cagiano, P. Bufo, E. Maiorano, D. Ribatti, E. Ranieri, M. Gigante, L. Gesualdo, M. Ferro, O. De Cobelli, C. Buonerba, G. Di Lorenzo, S. De Placido, S. Palazzo, C. Bettocchi, P. Ditonno, M. Battaglia. - In: MEDICINE. - ISSN 0025-7974. - 94:46(2015 Nov), pp. e2117.1-e2117.10.
Abstract:
Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase, was initially identified as the second glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. Later studies demonstrated that GPI was the same as the autocrine motility factor (AMF), and that it mediates its biological effects through the interaction with its surface receptor (AMFR/gp78). In this study, we assessed the role of GPI/AMF as a prognostic factor for clear cell renal cell carcinoma (ccRCC) cancerspecific (CSS) and progression-free survival (PFS). In addition, we evaluated the expression and localization of GPI/AMF and AMFR, using tissue microarray-based immunohistochemistry (TMA-IHC), indirect immunofluorescence (IF), and confocal microscopy analysis. Primary renal tumor and nonneoplastic tissues were collected from 180 patients who underwent nephrectomy for ccRCC. TMA-IHC and IF staining showed an increased signal for both GPI and AMFR in cancer cells, and their colocalization on plasma membrane. Kaplan-Meier curves showed significant differences in CSS and PFS among groups of patients with high versus low GPI expression. In particular, patients with high tissue levels of GPI had a 5-year survival rate of 58.8%, as compared to 92.1% for subjects with low levels (P<0.0001). Similar findings were observed for PFS (56.8% vs 93.3% at 5 years). At multivariate analysis, GPI was an independent adverse prognostic factor for CSS (HR1.26; P0.001), and PFS (HR1.16; P0.01). In conclusion, our data suggest that GPI could serve as a marker of ccRCC aggressiveness and a prognostic factor for CSS and PFS.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Carcinoma, Renal Cell; Cytokines; Female; Fluorescent Antibody Technique, Indirect; Glucose-6-Phosphate Isomerase; Humans; Immunohistochemistry; Kidney; Kidney Neoplasms; Male; Microscopy, Confocal; Middle Aged; Oligonucleotide Array Sequence Analysis; Prognosis; Real-Time Polymerase Chain Reaction; Retrospective Studies; Survival Analysis; Up-Regulation; Gene Expression Regulation, Neoplastic; Medicine (all)
Elenco autori:
G. Lucarelli, M. Rutigliano, F. Sanguedolce, V. Galleggiante, A. Giglio, S. Cagiano, P. Bufo, E. Maiorano, D. Ribatti, E. Ranieri, M. Gigante, L. Gesualdo, M. Ferro, O. De Cobelli, C. Buonerba, G. Di Lorenzo, S. De Placido, S. Palazzo, C. Bettocchi, P. Ditonno, M. Battaglia
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