The epithelial–mesenchymal transition (EMT) in equine amniotic multipotent progenitor cells induces changes of the cell glycan profile
Altro
Data di Pubblicazione:
2013
Citazione:
The epithelial–mesenchymal transition (EMT) in equine amniotic multipotent progenitor cells induces changes of the cell glycan profile / A. Lange-Consiglio, G. Accogli, F. Cremonesi, S. Desantis. ((Intervento presentato al 11. convegno Società Italiana di Riproduzione Animale tenutosi a Ustica nel 2013.
Abstract:
ABSTRACT - The glycoprotein pattern was investigated in equine amniotic epithelial, mesenchymal and transdifferentiated cells with a panel of twelve lectins, in combination with saponification and sialidase digestion (K-s). NeuNacα2,3Galβ1,4GlcNAc, NeuNAcα2,6Gal/GalNAc and GalNAc (MAL II, SNA, SBA affinity) were mainly expressed in the epithelial cells, less in the mesenchymal cells and even lesser in the transdifferentiated ones, whereas N-linked glycans from high-Man type glycans (Con A reactivity) showed an opposite trend. Fucosylated LTA-binding glycans were present in epithelial and mesenchymal cells but not in the transdifferentiated cells which, on the contrary, contained terminal L-Fucα1,2Galβ1,4GlcNAcβ and αGal residues (UEA I and GSA I-B4 binding). Interestingly, only transdifferentiated cells expressed NeuNacGalNAcα1,3(LFucα1,2)Galβ1,3/4GlcNAcβ1 and Galβ1,3GalNAc terminating glycans (K-s-DBA and PNA affinity). These findings show that the epithelial–mesenchymal transition (EMT) in equine amniotic multipotent progenitor cells is characterized by glycosylation changes which produce cell specific glycan profile.
INTRODUCTION - The epithelial to mesenchymal transition (EMT) is the process by which epithelial cells dramatically alter their shape and motile behavior as they differentiate into mesenchymal cells. During the EMT, epithelial cells lose their apical-basal polarity and extensive adhesions to neighboring cells and basement membranes. Coincident with the modification of cell-cell and cell substrate adhesions, the transitioning epithelial cell undergoes a dramatic shape change, adopting an extensively flattened and elongated leading-trailing mesenchymal morphology (Mendez et al., 2010).
The EMT and the reverse process, termed the mesenchymal-epithelial transition (MET), play central roles in embryogenesis (Thiery and Sleeman, 2006). For example, in the embryo itself, the first EMT event occurs at gastrulation. A subset of cells from the epiblast, the single epithelial cell layer of the embryo, move to the midline to form the primitive streak, a linear structure that bisects the embryo along the antero-posterior axis. These cells undergo EMT and internalize to generate mesoderm and endoderm, while those remaining in the epiblast become ectoderm. Thus, the embryo is transformed from a single layer to three germ layers. Mesoderm and endoderm contribute to many tissues of the adult organism by undergoing several rounds of EMT and MET. Primary EMT also occurs during vertebrate nervous system development to generate neural crest cells. The epithelial neural plate in the midline of the embryo rolls up to form the cylindrical neural tube, precursor to the brain and spinal cord. Those epithelial cells located near the dorsal midline of the neural tube undergo primary EMT to become migratory neural crest cells, which subsequently move away from the neural tube, navigating along stereotypic pathways throughout the embryo. (Le Douarin and Kalcheim, 1999). Primary EMT events such as gastrulation and formation of migratory neural crest cells are followed by the generation of distinct cell types.
Gastrulation and neural crest formation are two prototypical processes governed by EMT in amniotes. But, EMT-like process is also evoked during tumor progression and metastasis emergence where, as in embryogenesis, cells undergo EMT to migrate and colonize distant territories. While EMT and its converse MET, are concepts first defined by Elizabeth Hay about 40 years ago (Hay et al. 1968), the role of EMT-like process during tumor progression is usually not well understood. It is noteworthy that in placental mammals the epithelial layer of amnion originates from the trophectoderm and it is continuous with the epiblast, the
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
A. Lange-Consiglio, G. Accogli, F. Cremonesi, S. Desantis
Link alla scheda completa: