Sequence-specific amplified polymorphism (ssap) technique to analyse Fusarium oxysporum f. sp. lactucae vcg 0300 isolate from lettuce
Articolo
Data di Pubblicazione:
2008
Citazione:
Sequence-specific amplified polymorphism (ssap) technique to analyse Fusarium oxysporum f. sp. lactucae vcg 0300 isolate from lettuce / M. Pasquali, D. Saravanakumar, M.L. Gullino, A. Garibaldi. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - 90:3(2008), pp. 527-535. [10.4454/jpp.v90i3.697]
Abstract:
A sequence-specific amplified polymorphism (SSAP) technique was developed to study the clonality of Fusarium oxysporum f.sp. lactucae VCG 0300 which causes Fusarium wilt of lettuce. Specific primers designed from two known long terminal repeats (LTRs) of the F. oxysporum genome were coupled with MseI AFLP-derived primers to investigate the level of polymorphism in 80 isolates of F. oxysporum obtained from different locations worldwide. The technique proved to be reliable, efficient and able to discriminate among F. oxysporum f.sp. lactucae race 1 strains and a single strain of race 2. Polymorphisms within Italian and US race 1 isolates were detected but no relation was found between genetic variability and geographic distribution of strains. The technique confirmed the worldwide clonality of VCG 0300 race 1. In addition, the present study revealed that the SSAP technique can be used for reliable strain discrimination and as a tool to investigate transposition behaviour of retroelements in the genome of the pathogen.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Diagnostics; molecular characterization; long terminal repeats (LTRs); SSAP; Fusarium wilt; lettuce
Elenco autori:
M. Pasquali, D. Saravanakumar, M.L. Gullino, A. Garibaldi
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