Real-time polymerase chain reaction for identification of a highly pathogenic group of Fusarium oxysporum f.sp. chrysanthemi on argyranthemum frutescens L.
Articolo
Data di Pubblicazione:
2004
Citazione:
Real-time polymerase chain reaction for identification of a highly pathogenic group of Fusarium oxysporum f.sp. chrysanthemi on argyranthemum frutescens L / M. Pasquali, L. Marena, E. Fiora, P. Piatti, M.L. Gullino, A. Garibaldi. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - 86:1(2004), pp. 53-59.
Abstract:
A real-time Polymerase Chain Reaction (PCR) system based on Taqman® chemistry was developed in order to identify a new group of Fusarium oxysporum f.sp. chrysanthemi highly pathogenic on Paris daisy (VCG 0052). Sensitivity of PCR and real-time PCR was compared on genomic DNA from mycelium. The ability to identify symptomless infections on daisy was also evaluated using selective substrate, PCR and real-time PCR. Real-time PCR could identify infected plants on the fifth day after artificial inoculation, although plants remained symptomless until the 13th day after inoculation. The real-time PCR could detect as little as 300 fg of genomic DNA. The advantages of real-time PCR as a diagnostic system for this new epidemic disease are discussed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Taqman probe; selective substrate; PCR sensitivity; prevention; VCG 0052
Elenco autori:
M. Pasquali, L. Marena, E. Fiora, P. Piatti, M.L. Gullino, A. Garibaldi
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