Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection
Articolo
Data di Pubblicazione:
2008
Citazione:
Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection / M. Neupärtl, C. Meyer, I. Woll, F. Frohns, M. Kang, J.L. Van Etten, D. Kramer, B. Hertel, A. Moroni, G. Thiel. - In: VIROLOGY. - ISSN 0042-6822. - 372:2(2008), pp. 340-348.
Abstract:
Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K+ from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K+ efflux with a concomitant decrease in the intracellular K+. This K+ efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K+ efflux are at least partially mediated by Kcv. The virus-triggered K+ efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K+ and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chlorella viruses ; viral K+ channel Kcv ; K+ efflux ; Phycodnaviridae ; PBCV-1
Elenco autori:
M. Neupärtl, C. Meyer, I. Woll, F. Frohns, M. Kang, J.L. Van Etten, D. Kramer, B. Hertel, A. Moroni, G. Thiel
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