Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement
Articolo
Data di Pubblicazione:
2016
Citazione:
Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement / A. Altomare, L. Regazzoni, X.M.P. Parra, F. Selmin, C. Rumio, M. Carini, G. Aldini. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - 1028(2016), pp. 130-144. [10.1016/j.jchromb.2016.06.013]
Abstract:
A validated analytical procedure is here described for the quality control of the protein fraction of purified bovine colostrum used in food supplements. The proposed procedure starts with 1D and 2D-gel electrophoresis. The sample is then separated into two fractions by protein G affinity chromatography: the IgG enriched and the IgG depleted fraction (IgG-d). A size exclusion chromatography coupled to UV is then applied to the IgG and IgG-d fractions for the quantitative analysis of IgG and IgM, respectively. The IgG-d fraction is then analysed by HPLC-MS analysis for the quantitative analysis of beta-lactoglobulins and alpha-lactoalbumin. The next step is to quantitatively measure a set of bioactive proteins selected from the bovine colostrum data bank on the basis of their claimed health benefits. The enzymatic activities of lactoperoxidase and xanthine dehydrogenase/oxidase are then tested as an index of protein functionality.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bovine colostrum; Quality control; Food supplement; Size exclusion chromatography; Mass spectrometry
Elenco autori:
A. Altomare, L. Regazzoni, X.M.P. Parra, F. Selmin, C. Rumio, M. Carini, G. Aldini
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