Determination by UPLC/MS-MS of Coenzyme Q10 (CoQ10) in Plasma of Healthy Volunteers before and after Oral Intake of Food Supplements Containing CoQ10
Articolo
Data di Pubblicazione:
2015
Citazione:
Determination by UPLC/MS-MS of Coenzyme Q10 (CoQ10) in Plasma of Healthy Volunteers before and after Oral Intake of Food Supplements Containing CoQ10 / G.L. Visconti, L. Mazzoleni, C. Rusconi, V. Grazioli, G. Roda, G. Manini, V. Gambaro. - In: JOURNAL OF ANALYTICAL & BIOANALYTICAL TECHNIQUES. - ISSN 2155-9872. - 6:S13(2015), pp. 1-5. [10.4172/2155-9872.S13-011]
Abstract:
To evaluate CoQ10 concentration a UPLC/MS-MS method for its analysis in plasma samples was developed.
Before validation, the choice of the anticoagulant, of the Internal Standard (CoQ9; IS) and the stability of CoQ10
in plasma were evaluated. These determinations were necessary to assess the effect of the oral intake of CoQ10
in nine healthy volunteers after 10 days of treatment with a food supplement containing it. The basal levels of
CoQ10 were determined and the analyses were repeated after four days to take into account the variability of
endogenous substances. After oral intake, the increase in plasma concentration of CoQ10 resulted statistically
significant (P<0.05), indicating that the assumption of food supplements containing this ubiquinone could be of help
to increase its concentration, thus exploiting the its beneficial properties.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Coenzyme Q10; CoQ9; Plasma; Oral intake; UPLC/MSMS
Elenco autori:
G.L. Visconti, L. Mazzoleni, C. Rusconi, V. Grazioli, G. Roda, G. Manini, V. Gambaro
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