The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
Articolo
Data di Pubblicazione:
2016
Citazione:
The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA / A. Aviñó, S. Mazzini, R. Gargallo, R. Eritja. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 17:2(2016 Feb), pp. 211.1-211.13. [10.3390/ijms17020211]
Abstract:
Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
DNA; RNA; cosolute; molecular crowding; thermal stability; triplex forming oligonucleotide (TFO)
Elenco autori:
A. Aviñó, S. Mazzini, R. Gargallo, R. Eritja
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