Protein nanocrystallography : Growth mechanism and atomic structure of crystals induced by nanotemplates
Articolo
Data di Pubblicazione:
2005
Citazione:
Protein nanocrystallography : Growth mechanism and atomic structure of crystals induced by nanotemplates / E. Pechkova, F. Vasile, R. Spera, S. Fiordoro, C. Nicolini. - In: JOURNAL OF SYNCHROTRON RADIATION. - ISSN 0909-0495. - 12:6(2005 Nov), pp. 772-778. (Intervento presentato al convegno Workshop on Synchrotron Radiation and Nanobiosciences : September, 9-12 tenutosi a Porte Conte (Italy) nel 2004) [10.1107/S0909049505011647].
Abstract:
Protein nanocrystallography, a new technology for crystal growth based on protein nanotemplates, has recently been shown to produce diffracting, stable and radiation-resistant lysozyme crystals. This article, by computing these lysozyme crystals' atomic structures, obtained by the diffraction patterns of microfocused synchrotron radiation, provides a possible mechanism for this increased stability, namely a significant decrease in water content accompanied by a minor but significant α-helix increase. These data are shown to be compatible with the circular dichroism and two-dimensional Fourier transform spectra of high-resolution H NMR of proteins dissolved from the same nanotemplate-based crystal versus those from a classical crystal. Finally, evidence for protein direct transfer from the nanotemplate to the drop and the participation of the template proteins in crystal nucleation and growth is provided by high-resolution NMR spectrometry and mass spectrometry. Furthermore, the lysozyme nanotemplate appears stable up to 523 K, as confirmed by a thermal denaturation study using spectropolarimetry. The overall data suggest that heat-proof lysozyme presence in the crystal provides a possible explanation of the crystal's resistance to synchrotron radiation.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
CD; Mass spectroscopy; NMR; Protein crystals; Animals; Chickens; Crystallization; Magnetic Resonance Spectroscopy; Muramidase; Nanotechnology; Mass Spectrometry; Physics and Astronomy (miscellaneous); Instrumentation; Atomic and Molecular Physics, and Optics; Radiation
Elenco autori:
E. Pechkova, F. Vasile, R. Spera, S. Fiordoro, C. Nicolini
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