Investigation of corticosteroids profiles in bovine urine : part A: development of a method for cortisol,prednisolone and their metabolites determination
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Data di Pubblicazione:
2016
Citazione:
Investigation of corticosteroids profiles in bovine urine : part A: development of a method for cortisol,prednisolone and their metabolites determination / M. Belloni, F. Ostorero, M. Leporati, M.C. Abete, F. Arioli, G. Dusi, M. Gili. ((Intervento presentato al 8. convegno Residues of Veterinary Drugs in Food tenutosi a Egmond aan Zee nel 2016.
Abstract:
Prednisolone is a synthetic corticosteroid with anti-inflammatory and gluconeogenetic activities. Corticosteroids are used for the treatment of inflammatory diseases: for this reason, Commission Regulation 2010/37/EC established maximum residue levels (MRLs) for corticosteroids in liver and muscle; in urine no MRLs have been set therefore their presence at any concentration is not allowed. In recent years the prednisolone was frequently observed at residue level (concentrations < 5 μg/l) in bovine urine, especially in samples collected at the slaughterhouse, but also in farming; in all cases, no therapeutic use was declared in advance by farm veterinarians. Recent studies have confirmed the hypothesis that prednisolone can be formed in vivo by endogenous cortisol in animals subjected to conditions of acute (e.g.: transport and slaughter), or chronic (e.g.: chronic diseases, inflammatory, ill-treatment) stress. In 2012 the Italian Ministry of Health has set at 5 μg/l
the limit value for not compliant results; this provision should be temporary, pending on the identification of biomarkers reliable of exogenous origin of prednisolone. Thus, the interest for pattern recognition of corticosteroid metabolites profiles has been increased; the study of metabolites could be a promising approach that can be used to detect the abuse of these substances, in order to distinguish treated from non-treated animals. The aim of this study was the development of a accurate and robust LC-MS/MS procedure for simultaneous quantitative determination of 22 molecules (Table 1) correlated to prednisolone and cortisol metabolism in bovine urine; this method will be used to map the metabolites profile and investigate the alteration of endogenous pattern in case of stress or pharmacological treatment and therefore to obtain a powerful instrument to detect illicit operations.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
M. Belloni, F. Ostorero, M. Leporati, M.C. Abete, F. Arioli, G. Dusi, M. Gili
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