Estrogenic activity of procymidone in rainbow trout (Oncorhynchus mykiss) hepatocytes: a possibile mechanism of action
Articolo
Data di Pubblicazione:
2004
Citazione:
Estrogenic activity of procymidone in rainbow trout (Oncorhynchus mykiss) hepatocytes: a possibile mechanism of action / S. Radice, R. Fumagalli, E. Chiesara, M. Ferraris, S. Frigerio, L. Marabini. - In: CHEMICO-BIOLOGICAL INTERACTIONS. - ISSN 0009-2797. - 147:2(2004), pp. 185-193.
Abstract:
Procymidone modifies sexual differentiation in vitro and induces estrogenic activity in primary cultured rainbow trout hepatocytes, as shown
by an increase in the contents of vitellogenin and heat shock proteins. Since this dicarboximide fungicide is found in human tissues, it was
considered of interest to investigate its ability to induce endocrine damage in the MCF-7 human cell line. The mechanism of this estrogenic action
was also evaluated. Procymidone 100 AM stimulated cell growth from day 3 up to day 12 and raised the level of pS2 on day 3. Although
procymidone does not bind the estrogen receptor (ER), the antiestrogen ICI 182780 inhibited its effect on cell growth and pS2 content, suggesting
that the ER is involved indirectly in these effects. In exploring the mechanism of ER indirect activation we found that the antibody against c-Neu
receptor (9G6) did not modify procymidone’s effects on cell growth and pS2 expression. Thus, procymidone does not bind the c-Neu membrane
receptor, excluding this indirect ER activation pathway. We also found that procymidone induced mitogen-activated protein kinase (MAPK) at 15
and 30 min, and that PD 98059, a MAPK (Erk1/2) inhibitor, prevented procymidone’s effects on cell growth and pS2, indicating that MAPK
activation is responsible for procymidone ER activation. The production of reactive oxygen species (ROS) with these times and elimination of the
phenomenon by a-tocopherol (a-T), a ROS scavenger, is proof that oxygen free-radical production is at the basis of the MAPK activation by
procymidone.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
17β-estradiol; Androgen receptor; AR; E2; ER; Estrogenic receptor; Heat shock protein; HSP; MAPK; Mitogen-activated protein kinase; PBS; Phosphate buffered saline; Procymidone; Reactive oxygen species; ROS; Trout hepatocytes; Vitellogenin; Vtg
Elenco autori:
S. Radice, R. Fumagalli, E. Chiesara, M. Ferraris, S. Frigerio, L. Marabini
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