Data di Pubblicazione:
2016
Citazione:
Nasunin, a new player in the field of osteoblast protection against oxidative stress / L. Casati, F. Pagani, P.C. Braga, R. Lo Scalzo, V. Sibilia. - In: JOURNAL OF FUNCTIONAL FOODS. - ISSN 1756-4646. - 23:(2016 May), pp. 474-484. [10.1016/j.jff.2016.03.007]
Abstract:
This study investigated whether nasunin, the major component of anthocyanin pigment of eggplant (Solanum melongena L.), known for its antioxidant effects, counteracts tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in MC3T3-E1 osteoblastic cells. Pretreatment with purified nasunin (10-10-10-6 M) significantly increased viability and reduced apoptosis of MC3T3-E1 cells cultured with t-BHP (250 μM, 3 h). Furthermore, nasunin (10-9 M) prevented t-BHP-induced osteoblastic dysfunction and changes in the cytoskeleton organisation due to both an increase of intracellular glutathione and a decrease of intracellular reactive oxygen species. Moreover, t-BHP-induced reduction in osteoblast differentiation markers, such as alkaline phosphatase and collagen content, was recovered in nasunin-pretreated cells. Nasunin protects against t-BHP-induced osteoblastic dysfunction by activating the phosphoinositide 3-kinase/Akt signalling pathway (PI3K/Akt) since LY294002, a PI3K-specific inhibitor, worsened the cytotoxic effect of t-BHP and reversed the protective action of nasunin. Furthermore, nasunin prevented the decreased Akt phosphorylation observed in t-BHP-treated cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Nasunin; Osteoblastic dysfunction; Osteoblasts; Oxidative stress; Phosphoinositide 3-kinase/AKT pathway; Food Science; Medicine (miscellaneous); Nutrition and Dietetics
Elenco autori:
L. Casati, F. Pagani, P.C. Braga, R. Lo Scalzo, V. Sibilia
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