Endocannabinoid system regulates migration of endometrial stromal cells via cannabinoid receptor 1 through the activation of PI3K and ERK1/2 pathways
Articolo
Data di Pubblicazione:
2010
Citazione:
Endocannabinoid system regulates migration of endometrial stromal cells via cannabinoid receptor 1 through the activation of PI3K and ERK1/2 pathways / D. Gentilini, A. Besana, P. Vigano, P. Dalino, M. Vignali, M. Melandri, M. Busacca, A.M. Di Blasio. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - 93:8(2010), pp. 2588-2593. [10.1016/j.fertnstert.2010.02.006]
Abstract:
Objective: To evaluate the effects of the cannabinoid system on the regulation of endometrial stromal cell (ESCs) dynamic behavior.
Design: ESC migration, electrical signal generated by K(+) channels, and cytoskeletal-actin dynamics were evaluated in response to treatment with the synthetic endocannabinoid methanandamide. Selective agonists and antagonists were used to identify both the receptor and the biochemical pathways involved.
Setting: Molecular research institution.
Patient(s): Endometrial tissues were obtained from 40 reproductive-age women undergoing laparoscopy for benign pathologies.
Interventions: ESCs were treated with methanadamide and with selective agonist (ACEA) and antagonist (AM251) of the cannabinoid receptor 1.
Main Outcome Measures: Cellular migration was evaluated by means of chemotaxis experiments in a Boyden chamber. Electric signal generated by K(+) channels was evaluated by patch clamp experiments Cellular morphology and cytoskeletal-actin dynamics were evaluated by immunofluorescence.
Result(s): Methanandamide enhanced ESC migration via cannabinoid receptor I (CNR1) through the activation of PI3K/Akt and ERK1/2 pathways. The increased ESC migration was associated with cytoskeleton reorganization identified by the dissolution of F-actin stress fibers and the presence of stress fiber arcs and with increased electrical signal generated by K(+) channels.
Conclusion(s): In physiologic conditions, the cannabinoid system has a central role in regulating endometrial cell migration. The involvement of ERK1/2 and PI3-K/Akt pathways points to a potential role of endocannabinoids in some pathologic conditions characterized by enhanced endometrial cell invasiveness.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Endometrium; cellular migration; endocannabinoid system; methanandamide
Elenco autori:
D. Gentilini, A. Besana, P. Vigano, P. Dalino, M. Vignali, M. Melandri, M. Busacca, A.M. Di Blasio
Link alla scheda completa: