Dietary fish oil and stearate action on adipose lipid metabolism transcriptomics in periparturient dairy goats
Abstract
Data di Pubblicazione:
2015
Citazione:
Dietary fish oil and stearate action on adipose lipid metabolism transcriptomics in periparturient dairy goats / J.M. Caputo, G. Invernizzi, A. Campagnoli, G. Farina, M. Ferroni, A. Agazzi, J.J. Loor, G. Savoini. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - 14:suppl. 1(2015 Jun), pp. 72-72. ((Intervento presentato al 21. convegno ASPA Congress tenutosi a Milano nel 2015.
Abstract:
The aim of this study was to evaluate the effects of saturated or
unsaturated fatty acids on adipose mRNA level of genes involved
in the major lipogenic metabolic pathways. Twenty-three second
parity alpine dairy goats were assigned to three treatments: (C;
n=8) fed a non fat-supplemented basal diet, (ST; n=7) fed a
basal diet supplemented with stearic acid and (FO; n=8) fed a
basal diet supplemented with fish oil. The supplementation started
from the last week of gestation and lasted 21 days after kidding.
Treatments supplied 30g/head/d extra fatty acids during the
dry period and 50g/head/d during lactation. Individual blood samples
were taken at 14, 7 and 2 days before the expected kidding
day as well as 0, 2, 7, 14 and 21 DIM. Liver and adipose tissue
biopsies from each experimental subject were harvested on days
-7, +7 and +21 relative to parturition. Quantitative real-time RTPCR
of ADIPOQ, LPIN1, LPL, PPARG, SREBF1 and THRSP expression
was performed. Data obtained were analyzed using the
MIXED procedure of SAS. No differences were observed for milk
production, milk composition, body weight and body condition
score. Differences were detected for EB between C compared
with ST and FO at day 7 (0.34 vs. -1.12 and 0.91 respectively;
P≤0.05). Serum cholesterol concentration was significantly
higher in FO compared to C (P<0.01) and ST (P<0.05) at day 21.
Fat supplementation significantly affected subcutaneous adipose
genes involved in TAG synthesis (LPIN1), in the regulation of
lipogenesis (THRSP) and nuclear receptor (SREBF1). No effects
were observed for genes involved in LCFA uptake (LPL) and on
the expression of the adiponectin (ADIPOQ). LPIN1 expression
was significantly higher in the FO group compared with ST
(1.46-fold vs. 0.59-fold; P<0.04) suggesting increased lipogenesis.
THRSP (P=0.003) and SREBF1 (P=0.04) were up regulated
before kidding and increasingly down regulated from 7 to 21 days
postpartum. In conclusion, this study highlighted the important
role of saturated or unsaturated fat administration in dairy goat
diets to modulate mRNA expression of genes involved in TAG
synthesis, regulation of lipogenesis and nuclear receptor.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
J.M. Caputo, G. Invernizzi, A. Campagnoli, G. Farina, M. Ferroni, A. Agazzi, J.J. Loor, G. Savoini
Link alla scheda completa: