Single particle optical extinction and scattering allows real time quantitative characterization of drug payload and degradation of polymeric nanoparticles
Articolo
Data di Pubblicazione:
2015
Citazione:
Single particle optical extinction and scattering allows real time quantitative characterization of drug payload and degradation of polymeric nanoparticles / M. Potenza, T. Sanvito, S. Argentiere, C. Cella, B. Paroli, C. Lenardi, P. Milani. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 5(2015 Dec 15), pp. 18228.1-18228.9. [10.1038/srep18228]
Abstract:
The behavior of nanoparticles in biological systems is determined by their dimensions, size distribution,
shape, surface chemistry, density, drug loading and stability; the characterization of these parameters
in realistic conditions and the possibility to follow their evolution in vitro and in vivo are, in most of the
cases, far from the capabilities of the standard characterization technologies. Optical techniques such
as dynamic light scattering (DLS) are, in principle, well suited for in line characterization of nanoparticle,
however their fail in characterizing the evolution of nanoparticle in solution where change in particle
dimension and density is present. Here we present an in-line optical technique based on single particle
extinction and scattering (SPES) overcoming the limitations typical of DLS and allowing for the efficient
characterization of nanoparticle polydispersity, index of refraction and degradation dynamics in
solution. Using SPES, we characterized the evolution of PLGA nanoparticles with different structures
and drug payloads in solution and we compared the results with DLS. Our results suggest that SPES
could be used as a process analytical technology for pharmaceutical nanoparticle production.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
M. Potenza, T. Sanvito, S. Argentiere, C. Cella, B. Paroli, C. Lenardi, P. Milani
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