Data di Pubblicazione:
2014
Citazione:
Multi-spot, label-free detection of biomarkers in complex media by reflectionless surfaces / M. Salina, F. Giavazzi, E. Ceccarello, F. Damin, M. Chiari, M. Ciuffo, G.P. Accotto, M. Buscaglia. - In: PROCEDIA ENGINEERING. - ISSN 1877-7058. - 87(2014), pp. 58-61. ((Intervento presentato al 28. convegno European Conference on Solid-State Transducers, EUROSENSORS tenutosi a Brescia nel 2014.
Abstract:
The measurement of the intensity of light reflected by interfaces with extremely low reflectivity in water enables the label-free, multiplex quantification of the binding between immobilized probes (e.g. antibodies) and targets in solution using extremely simple instrumental set-up. Here we show that, despite its simplicity, the method enables label-free detection in complex samples characterized by high absorbance and turbidity. Diagnostic markers of Tomato spotted wilt virus are revealed in crude plant extracts of Datura stramonium leaves with early stage infections.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Immunoassay; Label-free; Optical biosensor; Reflective phantom interface; Tomato spotted wilt virus; Viral infection
Elenco autori:
M. Salina, F. Giavazzi, E. Ceccarello, F. Damin, M. Chiari, M. Ciuffo, G.P. Accotto, M. Buscaglia
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