Rapid bioassay-guided isolation of antibacterial clerodane type diterpenoid from Dodonaea viscosa (L.) Jaeq
Articolo
Data di Pubblicazione:
2015
Citazione:
Rapid bioassay-guided isolation of antibacterial clerodane type diterpenoid from Dodonaea viscosa (L.) Jaeq / M. Khurram, L.A. Lawton, C. Edwards, M. Iriti, A. Hameed, M.A. Khan, F.A. Khan, S.U. Rahman. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 16:9(2015 Aug 27), pp. 20290-20307.
Abstract:
Plant extracts are complex matrices and, although crude extracts are widely in use, purified compounds are pivotal in drug discovery. This study describes the application of automated preparative-HPLC combined with a rapid off-line bacterial bioassay, using reduction of a tetrazolium salt as an indicator of bacterial metabolism. This approach enabled the identification of fractions from Dodonaea viscosa that were active against Staphylococcus aureus and Escherichia coli, which, ultimately, resulted in the identification OPEN ACCESS of a clerodane type diterpenoid, 6β-hydroxy-15,16-epoxy-5β, 8β, 9β, 10α-cleroda-3, 13(16), 14-trien-18-oic acid, showing bacteriostatic activity (minimum inhibitory concentration (MIC) = 64–128 μg/mL) against test bacteria. To the best of our knowledge, this is the first report on antibacterial activity of this metabolite from D. viscosa.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Clerodane diterpenoid; Dodonaea viscosa; Preparative HPLC; XTT assay; Physical and Theoretical Chemistry; Organic Chemistry; Spectroscopy; Inorganic Chemistry; Catalysis; Molecular Biology; Computer Science Applications1707 Computer Vision and Pattern Recognition
Elenco autori:
M. Khurram, L.A. Lawton, C. Edwards, M. Iriti, A. Hameed, M.A. Khan, F.A. Khan, S.U. Rahman
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