Transplanted human adipose tissue-derived stem cells engraft and induce regeneration in mice olfactory neuroepithelium in response to dichlobenil subministration
Articolo
Data di Pubblicazione:
2014
Citazione:
Transplanted human adipose tissue-derived stem cells engraft and induce regeneration in mice olfactory neuroepithelium in response to dichlobenil subministration / V. Franceschini, S. Bettini, S. Pifferi, A. Menini, G. Siciliano, E. Ognio, A.T. Brini, E. Di Oto, R.P. Revoltella. - In: CHEMICAL SENSES. - ISSN 0379-864X. - 39:7(2014 Sep 07), pp. 617-629.
Abstract:
We used immunodeficient mice, whose dorsomedial olfactory region was permanently damaged by dichlobenil inoculation,
to test the neuroregenerative properties of transplanted human adipose tissue-derived stem cells after 30 and 60 days.
Analysis of polymerase chain reaction bands revealed that stem cells preferentially engrafted in the lesioned olfactory epithelium
compared with undamaged mucosa of untreated transplanted mice. Although basal cell proliferation in untransplanted
lesioned mice did not give rise to neuronal cells in the olfactory mucosa, we observed clusters of differentiating olfactory cells
in transplanted mice. After 30 days, and even more at 60 days, epithelial thickness was partially recovered to normal values,
as also the immunohistochemical properties. Functional reactivity to odorant stimulation was also confirmed through electroolfactogram
recording in the dorsomedial epithelium. Furthermore, we demonstrated that engrafted stem cells fused with
mouse cells in the olfactory organ, even if heterokaryons detected were too rare to hypothesize they directly repopulated
the lesioned epithelium. The data reported prove that the migrating transplanted stem cells were able to induce a neuroregenerative
process in a specific lesioned sensory area, enforcing the perspective that they could become an available tool for
stem cell therapy.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
herbicide; Nod-scid mice; olfactory mucosa; stem cell transplantation; tissue recovery
Elenco autori:
V. Franceschini, S. Bettini, S. Pifferi, A. Menini, G. Siciliano, E. Ognio, A.T. Brini, E. Di Oto, R.P. Revoltella
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