Oxidative stress and cell death are enhanced by N-3 pufa membrane incorporation in breast cancer cells
Abstract
Data di Pubblicazione:
2015
Citazione:
Oxidative stress and cell death are enhanced by N-3 pufa membrane incorporation in breast cancer cells / P.A. Corsetto, G. Montorfano, P. Roderi, G. Pani, S. Zava, I. Colombo, A.M. Rizzo. - In: THE FEBS JOURNAL. - ISSN 1742-4658. - 282:suppl. 1(2015), pp. 224-224. ((Intervento presentato al 40. convegno FEBS Congress : The Biochemical Basis of Life tenutosi a Berlin nel 2015.
Abstract:
Epidemiological studies highlight the correlation between the
long chain n-3 polyunsaturated fatty acids (n-3 PUFAs) and a
reduction of cancer, suggesting a protective n-3 PUFA effect.
The n-3 PUFAs have been shown to improve the efficacy of various
cancer prevention, chemotherapy drugs and radiation against
cancer. The potential mechanism comprises mainly alterations of
cellular lipid composition and metabolism, which might consequently
modulate membrane properties and functions, eicosanoid
productions, and modulation of different signaling pathways
related to cell growth and death. The goal of the study was to
investigate the effects of n-3 PUFA (EPA and DHA) incorporation
in two lines of human breast cancer cells characterized by
different expression of ER receptor. After treatments, PUFAs
were partially metabolized from both cell lines and were incorporated
in membrane phospholipids with different specificity, especially
in lipid rafts. The n-3 PUFA incorporation increased the
membrane unsaturation degree, decreased the sphingomyelin and
cholesterol content, and altered the Glutathione Peroxidase
(GPx), Reductase, Catalase activity, GSH and Malondialdehyde
(MDA) content. By using the annexin-V staining we confirmed
that DHA and EPA induce apoptosis and in addition we found
that caspase-8, an effector caspase, is activated in n-3 PUFA
treated cells. In conclusion we speculate that in breast cancer
cells DHA and EPA incorporation alters membrane organization
and might increase ROS accumulation in or near the plasma
membrane especially lipid rafts where the assembly of the death
inducing signaling complex (DISC) and the subsequent activation
of apoptosis take place.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
omega-3; breast cancer; lipid; oxidative stress
Elenco autori:
P.A. Corsetto, G. Montorfano, P. Roderi, G. Pani, S. Zava, I. Colombo, A.M. Rizzo
Link alla scheda completa: