Potassium transport in Actinidia deliciosa calluses cultured in the presence of 3-O-methylglucose
Abstract
Data di Pubblicazione:
1994
Citazione:
Potassium transport in Actinidia deliciosa calluses cultured in the presence of 3-O-methylglucose / N. Negrini, G.A. Sacchi, A. Alisi, A. Rivetta, M. Cocucci, S. Cocucci - In: Abstract VIII International Congress of Plant Tissue and Cell Culture[s.l] : Congress Plant Tissue and Cell Culture, 1994. - pp. 256-256 (( Intervento presentato al VIII. convegno International Congress of Plant Tissue and Cell Culture. tenutosi a Firenze nel 1994.
Abstract:
Calluses of Actinjdia dsliciosa were grown for successive subcultures on
Gamborg B5 medium supplemented with l0 g/l. sucrose and 40 g/l. 3- 0 -
melhylglucose (MEG). MEG was absorbed with a kinetics similar to that of glucose; its concenrration in the tissue rose quickly to a value similar
to that of tbc medium. A tropilic utilization of MEG was excluded
because no degradation to CO2 .was observed, even if some
metabolization of MEG look piace in the; calluses, as suggested by the
finding data, when 144C-MEG was administered to the callus,, about haif
of the ratdioactiivity was incorporated into a more polar than MEG. not
phosphorilated compound. Calluses grown in MEG showed water
osmotic potenlials more negative than those of the contro!, togheter with
higher organic osmolyte (sucrose and amino acids) conlents, while no
change in malate and gIucosc-6-phosphate levels was observed. 31p_
NMR shows that in MEG there was a grueat l incruse in a phosphorylated
mtabolile with a chemical shift similar to that of monophosphoesters
Altbough. far its permeabilily, MEG cannot be considered a classic
osmotic agent. il induces metabolic changes similar to those described for 0smotic adaptation. The dala suggest thal the observed adaptations might
be due to a physical or rnelabolic effect of MEG not linked to
difference between cell and medium 05motjc potential.
Tipologia IRIS:
03 - Contributo in volume
Elenco autori:
N. Negrini, G.A. Sacchi, A. Alisi, A. Rivetta, M. Cocucci, S. Cocucci
Link alla scheda completa:
Titolo del libro:
Abstract VIII International Congress of Plant Tissue and Cell Culture