Detection of cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
Articolo
Data di Pubblicazione:
2015
Citazione:
Detection of cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach / M. Marangi, A.V. Koehler, S.A. Zanzani, M.T. Manfredi, E. Brianti, A. Giangaspero, R.B. Gasser. - In: PARASITES & VECTORS. - ISSN 1756-3305. - 8:1(2015 May), pp. 274.1-274.5.
Abstract:
BACKGROUND:
Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates.
METHODS:
A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis.
RESULTS:
Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis.
CONCLUSIONS:
The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cyclospora; Genetic characterization; Mutation scanning-based sequencing; Non-human primates; Zoonosis
Elenco autori:
M. Marangi, A.V. Koehler, S.A. Zanzani, M.T. Manfredi, E. Brianti, A. Giangaspero, R.B. Gasser
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