Changes in the level of acid soluble tiols in plant tissues, as physiological markers of heavy metals stress.
Abstract
Data di Pubblicazione:
1996
Citazione:
Changes in the level of acid soluble tiols in plant tissues, as physiological markers of heavy metals stress / A. Rivetta, N. Negrini, M. Cocucci - In: International Conference on isoenzymes and molecular markers in plants : Basic and applied aspects / [a cura di] M. Sari Gorla, C Frova, R. Gianini,J. Scandalios, C. Schnarrenberg. - [s.l] : Celbio Duotech, 1996. - pp. 8-8 (( convegno International Conference on isoenzymes and molecular markers in plants : Basic and applied aspects tenutosi a villa Olmo Como nel 1996.
Abstract:
CHANGES IN THE LEVEL OF ACID SOLUBLE TRIOLS IN PLANT TISSUES, AS
PHYSIOLOGICAL MARKERS OF HEAVY METALS STRESS
A1berto Rivetta, Noemi Negrini and Maurizio Cocucci
Department of Plant Physiology and Soil Chemistry, University of Milan, Milan, Italy
The use of biological systems and particularly the changes in biochemical and physiological
parameters in plants could be an useful tool in monitoring polluted environments. It is known
that plants exposed to heavy metals can accumulate thiols, low molecular weight substances
rich in -SH groups. In this work, we consider the possibility to use the changes in the level of
acid soluble thiols, as physiological markers of heavy metals stress. The results indicated that
thiols level in organs of different plant species early and strongly increased in plants grown in
the presence of increasing Cd2+ concentrations,. Thiols level also increased in plants grown on
a standard substrate containing different amounts of exogenous added Cd2+ or heavy metals
polluted soil, although growth parameters such as fresh and dry weight, protein and
chlorophyll contents did not vary with respect to the controL In these two cases, when Cd2+ in
plants tissues reached the same level (30 nmol i' fw) the increase in thiols level was higher in
plants grown in the presence of polluted soil than Cd2+. These data suggest that the changes in
thiols level may be considered a physiological parameter of general plant stress and may be
easily used also in field conditions. Moreover, using glutathione affinity chromatography,
preliminary data suggest that changes in glutathione-dependent activities, in particular
glutathione transferase, may be used as very sensible markers of Cd2+ stress
Tipologia IRIS:
03 - Contributo in volume
Elenco autori:
A. Rivetta, N. Negrini, M. Cocucci
Link alla scheda completa:
Titolo del libro:
International Conference on isoenzymes and molecular markers in plants : Basic and applied aspects