Utility of blotting paper for serological tests to perform monitoring programs for European Brown Hare Syndrome (EBHS)
Abstract
Data di Pubblicazione:
2012
Citazione:
Utility of blotting paper for serological tests to perform monitoring programs for European Brown Hare Syndrome (EBHS) / M. Chiari, E. Gioia, N. Ferrari, L. Capucci, A. Lavazza - In: Joint 61. WDA / 10. Biennial EWDA conference Convergence in Wildlife Health : 22-27 July 2012, Lyon, France : proceedings[s.l] : Wildlife Disease Association & European Wildlife Disease Association, 2012 Jul 23. - pp. 384-384 (( Intervento presentato al 61. convegno International conference of the Wildlife Disease Association (WDA) / Biennial European Wildlife Disease Association (EWDA) conference tenutosi a Lyon (FR) nel 2012.
Abstract:
Since mid ‘80 the European brown hare (Lepus europeaus) populations have been
progressively declining due to several causes, including the occurrence of EBHS.
After the first outbreaks in North Italy in the ’90, the periodical EBHS cases imposed
the adoption of an articulate monitoring plan of the different hare populations,
including those from protected areas and the hunting territory. In addition to the
examination of dead animals for viral detection, such monitoring activity takes
advantage from serological survey i.e. by checking the presence of antibodies to
EBHSV. Since different types of blood sampling may be adopted according to each
situation, from 2005 to 2012, we planned to compare the serological titres obtained
by testing with cELISA: a) the “classical” serum b) samples of blood dried onto
blotting paper and c) bloody fluid from the heart cavities. The major aim was to
establish the utility of each sampling method for verifying hares’ health status and the
possibility to get data from low density areas, as hunting ones. We analysed the
following samples: a) + b = 305 animals; b) + c) = 182 animals; a) + c) = 95 animals.
Even if blotting paper and cardiac blood slightly underestimate the EBHSV antibody
titres, both these “alternative” sampling methods may be useful for field studies.
Moreover, the slightly underestimates of antibody titres do not prevent to correctly
interpret the sero-epidemiological results with regard to the understanding of
spatial/time exposure of the population to EBHS and the ability of single hares to
resist the EBHSV infection.
Tipologia IRIS:
03 - Contributo in volume
Elenco autori:
M. Chiari, E. Gioia, N. Ferrari, L. Capucci, A. Lavazza
Link alla scheda completa:
Titolo del libro:
Joint 61. WDA / 10. Biennial EWDA conference Convergence in Wildlife Health : 22-27 July 2012, Lyon, France : proceedings