Data di Pubblicazione:
2013
Citazione:
A simple method to generate adipose stem cell-derived neurons for screening purposes / C. Bossio, R. Mastrangelo, R. Morini, N. Tonna, S. Coco, C. Verderio, M. Matteoli, F. Bianco. - In: JOURNAL OF MOLECULAR NEUROSCIENCE. - ISSN 0895-8696. - 51:2(2013), pp. 274-281. [10.1007/s12031-013-9985-8]
Abstract:
Strategies involved in mesenchymal stem cell (MSC) differentiation toward neuronal cells for screening purposes are characterized by quality and quantity issues. Differentiated cells are often scarce with respect to starting undifferentiated population, and the differentiation process is usually quite long, with high risk of contamination and low yield efficiency. Here, we describe a novel simple method to induce direct differentiation of MSCs into neuronal cells, without neurosphere formation. Differentiated cells are characterized by clear morphological changes, expression of neuronal specific markers, showing functional response to depolarizing stimuli and electrophysiological properties similar to those of developing neurons. The method described here represents a valuable tool for future strategies aimed at personalized screening of therapeutic agents in vitro.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
differentiation; mesenchymal stem cells; neurons; adipocytes; animals; biological markers; calcium; female; mesenchymal stromal cells; neurons; primary cell culture; rats; rats, Sprague-Dawley; neurogenesis; cellular and molecular neuroscience
Elenco autori:
C. Bossio, R. Mastrangelo, R. Morini, N. Tonna, S. Coco, C. Verderio, M. Matteoli, F. Bianco
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