Data di Pubblicazione:
2013
Citazione:
A chitosan-based multilayered scaffold for multi-tissue regeneration / E. Varoni, J. Xu, A. Cochis, H. Chin, L. Altomare, G. Lodi, D.N. L, A. Carrassi, R. Chiesa, M. Cerruti, L. Rimondini. ((Intervento presentato al convegno 4th CESB Symposium on Biomaterials in Regenerative Medicine tenutosi a Sorrento nel 2013.
Abstract:
Orthopedic tissue engineering usually refers to the restitutio ad integrum of different
but uninterrupted regions (ligament, fibrocartilage, bone and skin) and it often employs
scaffolds to promote regeneration. Ideally, a scaffold should acts as clot retention
device, space maintainer, with a fine-tuned structure in harmony with surrounding
tissues. Our aim was the synthesis of a resorbable, sponge-like and multi-layered
chitosan scaffold, suitable for contextual multi-tissue regeneration.
A porous and multi-layered scaffold was produced by cross-linking chitosan with
genepin and further lyophilization. Chitosans with different molecular weights were
exploited in order to modulate the properties of the multi-layered structure: an interface
for cutaneous side was made of low molecular weight (LMW) chitosan, while a layer
for bone was composed of medium molecular weight (MMW) chitosan. A specific
structure with oriented pores, for ligament fibers, was obtained by MMW chitosan
electrodeposition.
A three-layered highly porous, sponge-like and cross-linked structure was obtained.
The MMW chitosan layer, for bone interface, revealed a better stability at in vitro
degradation test, in presence or not of lysozime, and an increased resistance to
compression. The oriented pore structure for ligament was synthesized. Different pore
sizes could be produced. All parts of the construct were cytocompatible.
The scaffold displayed tunable structure and physical-mechanical properties in
harmony with different tissues to be regenerated, suggesting a promising approach for
multi-tissue regeneration.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
E. Varoni, J. Xu, A. Cochis, H. Chin, L. Altomare, G. Lodi, D.N. L, A. Carrassi, R. Chiesa, M. Cerruti, L. Rimondini
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