Systemic inflammation alters satellite glial cell function and structure : a possible contribution to pain
Articolo
Data di Pubblicazione:
2014
Citazione:
Systemic inflammation alters satellite glial cell function and structure : a possible contribution to pain / E. Blum, P. Procacci, V. Conte, M. Hanani. - In: NEUROSCIENCE. - ISSN 0306-4522. - 274(2014 Aug 22), pp. 209-217. [10.1016/j.neuroscience.2014.05.029]
Abstract:
Local peripheral injury activates satellite glial cells (SGCs) in sensory ganglia, which may contribute to chronic pain. We hypothesized that systemic inflammation affects sensory ganglia like local injury. We induced systemic inflammation in mice by injecting lipopolysaccharide (LPS) intraperitoneally, and characterized SGCs and neurons in dorsal root ganglia (DRG), using dye injection, calcium imaging, electron microscopy (EM), immunohistochemistry, and electrical recordings. Several days post-LPS, SGCs were activated, and dye coupling among SGCs increased 3-4.5-fold. EM showed abnormal growth of SGC processes and the formation of new gap junctions. Sensitivity of SGCs to ATP increased twofold, and neuronal excitability was augmented. Blocking gap junctions reduced pain behavior in LPS-treated mice. Thus, changes in DRG due to systemic inflammation are similar to those due to local injury, which may explain the pain in sickness behavior and in other systemic diseases.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
satellite glial cell; dorsal root ganglion; purinergic receptors; gap junction; glial activation; sickness behavior
Elenco autori:
E. Blum, P. Procacci, V. Conte, M. Hanani
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