Data di Pubblicazione:
2012
Citazione:
2D DIGE analysis of protein extracts from muscle tissue / C. Gelfi, S. De Palma (METHODS IN MOLECULAR BIOLOGY). - In: Difference Gel Electrophoresis (DIGE) : methods and protocols / [a cura di] R. Cramer, R. Westermeier. - New York : Humana Press, 2012. - ISBN 9781617795725. - pp. 155-168 [10.1007/978-1-61779-573-2_11]
Abstract:
2D DIGE, two-dimensional difference gel electrophoresis, is a technology used to study the protein expression on two-dimensional gels. Protein samples are labeled with different color fluorescent dyes designed not to affect the relative migration of proteins during electrophoresis. Here, we describe the practical procedures necessary to perform a 2D DIGE experiment for a muscle tissue protein extract followed by CyDye DIGE fluors minimal labeling and the analysis of 2D DIGE gels for the assessment of quantitative differences.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
2D DIGE; 2D electrophoresis; CyDye DIGE fluors; Differential analysis; Muscle proteome
Elenco autori:
C. Gelfi, S. De Palma
Link alla scheda completa:
Titolo del libro:
Difference Gel Electrophoresis (DIGE) : methods and protocols