In vitro effects of zearalenone metabolites on cell proliferation and steroidogenesis with bovine granulosa cells
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Data di Pubblicazione:
2014
Citazione:
In vitro effects of zearalenone metabolites on cell proliferation and steroidogenesis
with bovine granulosa cells / F. Pizzo, F. Caloni, M. Albonico, N. Schreiber, M. Totty, L.J. Spicer. ((Intervento presentato al 18. convegno International Congress on In vitro Toxicology tenutosi a Egmond aan Zee nel 2014.
Abstract:
Zearalenone (ZEA), an oestrogenic fusariotoxin common contaminant of feedstuffs,
is rapidly metabolized in rumen into α-Zearalenol (α-ZEA) and to a lower amount
β-Zearalenol (β-ZEA). The purpose of this study is to evaluate the in vitro effects of
α-ZEA and β-ZEA on cell proliferation and steroidogenesis by using bovine granulosa
cells (GC). Ovaries were collected from a nearby slaughterhouse and small granulosa
cells (SMGC) were obtained by aspirating ovarian follicles (1-5 mm). Cells were cultured
for 2 days in 10% fetal bovine serum-containing medium followed by 2 days in serumfree
medium containing control or mycotoxin treatments. Cell numbers were determined
using a Coulter counter while concentrations of progesterone (P4) and estradiol (E2) in
cell culture medium were determined using radioimmunoassay. α-ZEA and β-ZEA were
tested at 0.31 μM and at 0.31, 3.1, 31 μM, respectively. A co-exposure to α-ZEA and
β-ZEA at 0.31 μM each was also carried out. All experiments were performed with and
without IGF1. Cell proliferation was inhibited in presence of IGF1 at 3.1 and 31 μM of
β-ZEA while without IGF1 only at 31 μM of β-ZEA was inhibitory. A synergic inhibitory
effect on cell proliferation and E2 production was demonstrated after co-exposure to
α-ZEA and β-ZEA only in cells treated with IGF1. Without IGF1, E2 production was
strongly stimulated at 31 μM compared to the control whereas in presence of IGF1 E2
release was inhibited (by 40%) by β-ZEA at 3.1 μM. P4 production was stimulated after
exposure to β-ZEA at the highest dose tested (31 μM) in presence and absence of IGF1.
Further studies are in progress to better understand the mechanism of action of ZEA and
its metabolites on GC.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
F. Pizzo, F. Caloni, M. Albonico, N. Schreiber, M. Totty, L.J. Spicer
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