Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems
Articolo
Data di Pubblicazione:
2015
Citazione:
Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems / M. Apparício, G.Q. Mostachio, T.F. Motheo, A.E. Alves, L. Padilha, E.A. Pires-Butler, P.A.P. Savi, R.A.R. Uscategui, G.C. Luvoni, W.R.R. Vicente. - In: REPRODUCTION FERTILITY AND DEVELOPMENT. - ISSN 1031-3613. - 27:7(2015), pp. 1082-1087. [10.1071/RD14022]
Abstract:
The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL-1 human chorionic gonadotrophin (hCG), 1?gmL-1 progesterone (P4) and 1?gmL-1 oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (PP<0.001). The stage of the oestrous cycle did not influence maturation rates.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dog; hormones; in vitro maturation
Elenco autori:
M. Apparício, G.Q. Mostachio, T.F. Motheo, A.E. Alves, L. Padilha, E.A. Pires Butler, P.A.P. Savi, R.A.R. Uscategui, G.C. Luvoni, W.R.R. Vicente
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