Data di Pubblicazione:
1998
Citazione:
NF-Y organizes the gamma-globin CCAAT boxes region / C. Liberati, A. Ronchi, P. Lievens, S. Ottolenghi, R. Mantovani. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 273:27(1998 Jul 03), pp. 16880-16889.
Abstract:
The CCAAT box is an important element in eukaryotic promoters and NF-Y (CBF) is a conserved heterotrimeric protein binding to it. Two subunits, NF-YB and NF-YC, contain a histone-like motif. We cloned the complete cDNA coding for the human NF-YC gene. The ORF codes for a 335 aa protein that shows virtual identity to the rat sequence, confirming the stunning invariance of NF-Y genes across species. We expressed and purified the yeast homology domain of NF-YC in bacteria and performed EMSA together with the corresponding conserved domains of NF-YA and NF-YB, obtaining a CCAAT-binding mini-NF-Y. We evaluated the expression of NF-YC and found that mRNA levels are similar in different human tissues except in testis.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Base Sequence ; CCAAT-Enhancer-Binding Proteins ; DNA, Recombinant ; DNA-Binding Proteins ; Globins ; Molecular Sequence Data ; Protein Binding ; Transcription Factors
Elenco autori:
C. Liberati, A. Ronchi, P. Lievens, S. Ottolenghi, R. Mantovani
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