Data di Pubblicazione:
1994
Citazione:
Dominant negative analogs of NF-YA / R. Mantovani, X.Y. Li, U. Pessara, R. Hooft van Huisjduijnen, C. Benoist, D. Mathis. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 269:32(1994 Aug 12), pp. 20340-20346.
Abstract:
NF-Y is a highly conserved heteromeric CCAAT-binding transcription factor involved in the function of several promoters. The NF-YA subunit contains a domain of high homology to yeast HAP2, which we show to be necessary and sufficient to mediate interactions with the NF-YB subunit and with DNA. Using protein affinity columns derivatized with amino acid substitution mutants, we further dissect this region into two functionally separable subdomains. The subunit association function resides in a 21-amino acid stretch, which is almost perfectly conserved among different species, while interaction with DNA resides in another short segment. We also show that DNA-binding mutants act as dominant repressors of NF-Y·DNA complex formation and of NF-Y- dependent transcription.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Genes, Dominant ; Amino Acid Sequence ; Base Sequence ; CCAAT-Enhancer-Binding Proteins ; DNA ; DNA-Binding Proteins ; Molecular Sequence Data ; Mutation ; Nuclear Proteins ; Oligodeoxyribonucleotides ; Sequence Alignment ; Structure-Activity Relationship ; Transcription Factors
Elenco autori:
R. Mantovani, X.Y. Li, U. Pessara, R. Hooft van Huisjduijnen, C. Benoist, D. Mathis
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