Comparative functional analysis of full-length and N-terminal fragments of phytochrome C, D and E in red light-induced signaling
Articolo
Data di Pubblicazione:
2013
Citazione:
Comparative functional analysis of full-length and N-terminal fragments of phytochrome C, D and E in red light-induced signaling / É. Ádám, S. Kircher, P. Liu, Z. Mérai, N. González-Schain, M. Hörner, A. Viczián, E. Monte, R.A. Sharrock, E. Schäfer, F. Nagy. - In: NEW PHYTOLOGIST. - ISSN 0028-646X. - 200:1(2013 Oct), pp. 86-96. [10.1111/nph.12364]
Abstract:
Phytochromes (phy) C, D and E are involved in the regulation of red/far-red light-induced photomorphogenesis of Arabidopsis thaliana, but only limited data are available on the mode of action and biological function of these lesser studied phytochrome species. We fused N-terminal fragments or full-length PHYC, D and E to YELLOW FLUORESCENT PROTEIN (YFP), and analyzed the function, stability and intracellular distribution of these fusion proteins in planta. The activity of the constitutively nuclear-localized homodimers of N-terminal fragments was comparable with that of full-length PHYC, D, E-YFP, and resulted in the regulation of various red light-induced photomorphogenic responses in the studied genetic backgrounds. PHYE-YFP was active in the absence of phyB and phyD, and PHYE-YFP controlled responses, as well as accumulation, of the fusion protein in the nuclei, was saturated at low fluence rates of red light and did not require functional FAR-RED ELONGATED HYPOCOTYL1 (FHY-1) and FHY-1-like proteins. Our data suggest that PHYC-YFP, PHYD-YFP and PHYE-YFP fusion proteins, as well as their truncated N-terminal derivatives, are biologically active in the modulation of red light-regulated photomorphogenesis. We propose that PHYE-YFP can function as a homodimer and that low-fluence red light-induced translocation of phyE and phyA into the nuclei is mediated by different molecular mechanisms.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Nuclear body formation; Nuclear translocation; Photomorphogenesis; Photoreceptor; Phytochrome E
Elenco autori:
É. Ádám, S. Kircher, P. Liu, Z. Mérai, N. González-Schain, M. Hörner, A. Viczián, E. Monte, R.A. Sharrock, E. Schäfer, F. Nagy
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