A protein microarray immunoassay for the serological evaluation of the antibody response in vertically transmitted infections
Articolo
Data di Pubblicazione:
2009
Citazione:
A protein microarray immunoassay for the serological evaluation of the antibody response in vertically transmitted infections / A. Ardizzoni, B. Capuccini, M.C. Baschieri, C.F. Orsi, F. Rumpianesi, S. Peppoloni, C. Cermelli, M. Meacci, A. Crisanti, P. Steensgaard, E. Blasi. - In: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. - ISSN 0934-9723. - 28:9(2009 Sep), pp. 1067-1075. [10.1007/s10096-009-0748-y]
Abstract:
The detection of specific serum antibodies is mainly achieved by enzyme-linked immunosorbent assay (ELISA). Here, we describe the setting up of a microarray-based serological assay to screen for IgG and IgM against vertically transmitted pathogens (Toxoplasma gondii, rubella virus, cytomegalovirus, herpes simplex virus types 1 and 2, varicella zoster virus, Chlamydia trachomatis). The test, accommodated onto a restricted area of a microscope slide, consists of: (a) the immobilization of antigens and human IgG and IgM antibody dilution curves, laid down in an orderly manner; (b) addition of serum samples; (c) detection of antigen-serum antibodies complexes by indirect immunofluorescence. The IgG and IgM curves provide an internal calibration system for the interpolation of the signals from the single antigens. The test was optimized in terms of spotting conditions and processing protocol. The detection limit was 400 fg for the IgG assay and 40 fg for the IgM assay; the analytical specificity was >98%. The clinical sensitivity returned an average value of 78%, the clinical specificity was >96%, the predictive values were >73%, and the efficiency was >88%. The results obtained make this test a promising tool, suitable for introduction in the clinical diagnostic routine of vertically transmitted infections, in parallel (and in future as an alternative) to ELISA.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Infectious Disease Transmission, Vertical ; Antibodies, Bacterial ; Antibodies, Protozoan ; Antibodies, Viral ; Communicable Diseases ; Female ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Pregnancy ; Protein Array Analysis ; Sensitivity and Specificity
Elenco autori:
A. Ardizzoni, B. Capuccini, M.C. Baschieri, C.F. Orsi, F. Rumpianesi, S. Peppoloni, C. Cermelli, M. Meacci, A. Crisanti, P. Steensgaard, E. Blasi
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