Data di Pubblicazione:
2014
Citazione:
Characterization of articular chondrocytes isolated from 211 osteoarthritic patients / M. Laganà, C. Arrigoni, S. Lopa, V. Sansone, L. Zagra, M. Moretti, M. Raimondi. - In: CELL AND TISSUE BANKING. - ISSN 1389-9333. - 15:1(2014 Mar), pp. 59-66. [10.1007/s10561-013-9371-3]
Abstract:
We analyzed specific features of chondrocytes
as cellular yield, cell doubling rates and the dependence between these parameters and patient-related data in a set of 211 osteoarthritic (OA) patients
undergoing total joint replacement. For each patient
the data available were joint type, age and gender.
Knee samples chosen randomly among all biopsies
were graded according to ICRS score. Patients’ age
ranged between 30 and 90 years with a mean age of
66 ± 9.7 years. Patients were divided into age classes
and statistically significant differences in proliferation
rate at passage 1 were found between chondrocytes
derived from young and old donors, with the last ones
characterized by a lower proliferation rate. A similar
trend was observed for proliferation rate at passage 2.
For all the samples, cellular yields ranged between 0.1
and 5.5 million cells/g of tissue. No significant correlation
was observed between the level of cartilage
degeneration (ICRS score) and cellular yield and
proliferation rates. However, in samples with a high
degree of cartilage degeneration (ICRS score 4) the
cellular yield was lower compared to the other three
groups (ICRS scores 1–3). In this study we performed a
systematic characterization of basic parameters of
chondrocytes originating from a wide group of OA
patients. Considering the use of autologous chondrocytes in chondral treatments, the characterization of cell basic features may represent an important step to determine the quality of the cell source which is a major determinant in the outcome of cell-based therapies.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Osteoarthritis ; Articular chondrocyte ; Age ; Yield ; ICRS score
Elenco autori:
M. Laganà, C. Arrigoni, S. Lopa, V. Sansone, L. Zagra, M. Moretti, M. Raimondi
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