Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis
Articolo
Data di Pubblicazione:
1985
Citazione:
Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis / L. Polonelli, G. Morace. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 21:1(1985 Jan), pp. 138-139.
Abstract:
Hybridoma cells produced by fusing myeloma cells with spleen cells from mice immunized with a soluble antigen of Microsporum canis yielded 30 antibody-producing clones. Six of these clones, propagated as ascites tumors in mice, showed two different types of monoclonal antibodies. The type 1 monoclonal antibody reacted with 17 heterologous and 10 homologous dermatophyte antigens. Type 2 monoclonal antibodies were unable to precipitate three antigens from different isolates of M. canis, thus suggesting the occurrence of different serotypes within the species.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
animals ; antibodies, fungal ; antibodies, monoclonal ; antigens, fungal ; cell line ; enzyme-linked immunosorbent assay ; hybridomas ; immunodiffusion ; mice ; mice, inbred BALB C ; microsporum ; serotyping ; spleen
Elenco autori:
L. Polonelli, G. Morace
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