Insights into the catalytic mechanism of glutathione S-transferase: the lesson from Schistosoma haematobium
Articolo
Data di Pubblicazione:
2005
Citazione:
Insights into the catalytic mechanism of glutathione S-transferase: the lesson from Schistosoma haematobium / F. Angelucci, P. Baiocco, M. Brunori, L. Gourlay, V. Morea, A. Bellelli. - In: STRUCTURE. - ISSN 0969-2126. - 13:9(2005), pp. 1241-1246. [10.1016/j.str.2005.06.007]
Abstract:
Glutathione S-transferases (GSTs) are involved in detoxification of xenobiotic compounds and in the biosynthesis of important metabolites. All GSTs activate glutathione (GSH) to GS-; in many GSTs, this is accomplished by a Tyr at H-bonding distance from the sulfur of GSH. The high-resolution structure of GST from Schistosoma haematobium revealed that the catalytic Tyr occupies two alternative positions, one external, involving a π-cation interaction with the conserved Arg21, and the other inside the GSH binding site. The interaction with Arg21 lowers the pKa of the catalytic Tyr10, as required for catalysis. Examination of several other GST structures revealed the presence of an external pocket that may accommodate the catalytic Tyr, and suggested that the change in conformation and acidic properties of the catalytic Tyr may be shared by other GSTs. Arginine and two other residues of the external pocket constitute a conserved structural motif, clearly identified by sequence comparison.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Animals ; Arginine ; Catalysis ; Enzyme Activation ; Glutathione Transferase ; Hydrogen Bonding ; Protein Conformation ; Schistosoma haematobium ; Tyrosine
Elenco autori:
F. Angelucci, P. Baiocco, M. Brunori, L. Gourlay, V. Morea, A. Bellelli
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