Data di Pubblicazione:
2013
Citazione:
A structure-based strategy for epitope discovery in Burkholderia pseudomallei OppA antigen / P. Lassaux, C. Peri, M. Ferrer-Navarro, L.J. Gourlay, A. Gori, O. Conchillo-Solé, D. Rinchai, G. Lertmemongkolchai, R. Longhi, X. Daura, G. Colombo, M. Bolognesi. - In: STRUCTURE. - ISSN 0969-2126. - 21:1(2013), pp. 167-175. [10.1016/j.str.2012.10.005]
Abstract:
We present an approach integrating structural and computational biology with immunological tests to identify epitopes in the OppA antigen from the Gram-negative pathogen Burkholderia pseudomallei, the etiological agent of melioidosis. The crystal structure of OppA(Bp), reported here at 2.1 angstrom resolution, was the basis for a computational analysis that identified three potential epitopes. In parallel, antigen proteolysis and immunocapturing allowed us to identify three additional peptides. All six potential epitopes were synthesized as free peptides and tested for their immunoreactivity against sera from healthy seronegative, healthy seropositive, and recovered melioidosis patients. Three synthetic peptides allowed the different patient groups to be distinguished, underlining the potential of this approach. Extension of the computational analysis, including energy-based decomposition methods, allowed rationalizing results of the predictive analyses and the immunocapture epitope mapping. Our results illustrate a structure-based epitope discovery process, whose application may expand our perspectives in the diagnostic and vaccine design fields.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Epitope Mapping ; Amino Acid Sequence ; Animals ; Antibodies, Bacterial ; Antigens, Bacterial ; Bacterial Proteins ; Burkholderia pseudomallei ; Carrier Proteins ; Computer Simulation ; Crystallography, X-Ray ; Female; Humans ; Immune Sera ; Lipoproteins ; Melioidosis ; Mice ; Mice, Inbred BALB C ; Models, Molecular ; Molecular Sequence Data ; Peptide Fragments ; Protein Binding ; Protein Structure, Secondary ; Protein Structure, Tertiary
Elenco autori:
P. Lassaux, C. Peri, M. Ferrer Navarro, L.J. Gourlay, A. Gori, O. Conchillo Solé, D. Rinchai, G. Lertmemongkolchai, R. Longhi, X. Daura, G. Colombo, M. Bolognesi
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