Targeting of Cameleons to various subcellular compartments reveals a strict cytoplasmic/mitochondrial Ca²⁺ handling relationship in plant cells
Articolo
Data di Pubblicazione:
2012
Citazione:
Targeting of Cameleons to various subcellular compartments reveals a strict cytoplasmic/mitochondrial Ca²⁺ handling relationship in plant cells / G. Loro, I. Drago, T. Pozzan, F. Lo Schiavo, M. Zottini, A. Costa. - In: PLANT JOURNAL. - ISSN 0960-7412. - 71:1(2012), pp. 1-13. [10.1111/j.1365-313X.2012.04968.x]
Abstract:
Here we describe use of a mitochondrial targeted Cameleon to produce stably transformed Arabidopsis plants that enable analyses of mitochondrial Ca 2+ dynamics in planta and allow monitoring of the intra-mitochondrial Ca 2+ concentration in response to physiological or environmental stimuli. Transgenic plants co-expressing nuclear and mitochondrial targeted Cameleons were also generated and analyzed. Here we show that mitochondrial Ca 2+ accumulation is strictly related to the intensity of the cytoplasmic Ca 2+ increase, demonstrating a tight association between mitochondrial and cytoplasmic Ca 2+ dynamics. However, under all experimental conditions, mitochondrial Ca 2+ dynamics were substantially different from those monitored in the cytoplasm, demonstrating that mitochondria do not passively sense cytosolic Ca 2+, but actively modulate the intra-mitochondrial level of the cation. In particular, our analyses show that the kinetics of Ca 2+ release from mitochondria are much slower than in the cytoplasm and nucleus. The mechanisms and functional implications of these differences are discussed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
plant mitochondria; Cameleon; Ca2+; imaging analyses; guard cells; root tip; Arabidopsis thaliana; technical advance
Elenco autori:
G. Loro, I. Drago, T. Pozzan, F. Lo Schiavo, M. Zottini, A. Costa
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