Calcium Dynamics in Root Cells of Arabidopsis thaliana Visualized with Selective Plane Illumination Microscopy
Articolo
Data di Pubblicazione:
2013
Citazione:
Calcium Dynamics in Root Cells of Arabidopsis thaliana Visualized with Selective Plane Illumination Microscopy / A. Costa, A. Candeo, L. Fieramonti, G. Valentini, A. Bassi. - In: PLOS ONE. - ISSN 1932-6203. - 8:10(2013), pp. e75646.1-e75646.11.
Abstract:
Selective Plane Illumination Microscopy (SPIM) is an imaging technique particularly suited for long term in-vivo analysis of transparent specimens, able to visualize small organs or entire organisms, at cellular and eventually even subcellular resolution. Here we report the application of SPIM in Calcium imaging based on Forster Resonance Energy Transfer (FRET). Transgenic Arabidopsis plants expressing the genetically encoded-FRET-based Ca2+ probe Cameleon, in the cytosol or nucleus, were used to demonstrate that SPIM enables ratiometric fluorescence imaging at high spatial and temporal resolution, both at tissue and single cell level. The SPIM-FRET technique enabled us to follow nuclear and cytosolic Ca2+ dynamics in Arabidopsis root tip cells, deep inside the organ, in response to different stimuli. A relevant physiological phenomenon, namely Ca2+ signal percolation, predicted in previous studies, has been directly visualized.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Costa, A. Candeo, L. Fieramonti, G. Valentini, A. Bassi
Link alla scheda completa: