Data di Pubblicazione:
2014
Citazione:
ACA12 is a deregulated isoform of plasma membrane Ca2+-ATPase of Arabidopsis thaliana / M. Limonta, S. Romanowsky, C. Olivari, M.C. Bonza, L. Luoni, A. Rosenberg, J.F. Harper, M.I. De Michelis. - In: PLANT MOLECULAR BIOLOGY. - ISSN 0167-4412. - 84:4-5(2014 Mar), pp. 387-397. [10.1007/s11103-013-0138-9]
Abstract:
Plant auto-inhibited Ca2+-ATPases (ACA)
are crucial in defining the shape of calcium transients and
therefore in eliciting plant responses to various stimuli.
Arabidopsis thaliana genome encodes ten ACA isoforms
that can be divided into four clusters based on gene structure
and sequence homology. While isoforms from clusters
1, 2 and 4 have been characterized, virtually nothing is
known about members of cluster 3 (ACA12 and ACA13).
Here we show that a GFP-tagged ACA12 localizes at the
plasma membrane and that expression of ACA12 rescues
the phenotype of partial male sterility of a null mutant
of the plasma membrane isoform ACA9, thus providing
genetic evidence that ACA12 is a functional plasma
membrane-resident Ca2+-ATPase. By ACA12 expression
in yeast and purification by CaM-affinity chromatography,
we show that, unlike other ACAs, the activity of ACA12
is not stimulated by CaM. Moreover, full length ACA12 is
able to rescue a yeast mutant deficient in calcium pumps.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arabidopsis thaliana; Ca2+-ATPase; Calmodulin; Plasma membrane
Elenco autori:
M. Limonta, S. Romanowsky, C. Olivari, M.C. Bonza, L. Luoni, A. Rosenberg, J.F. Harper, M.I. De Michelis
Link alla scheda completa: