The IGF-I/IGFBP-3 system in gingival crevicular fluid and dependence on application of fixed force
Articolo
Data di Pubblicazione:
2005
Citazione:
The IGF-I/IGFBP-3 system in gingival crevicular fluid and dependence on application of fixed force / M. Toia, R. Galazzo, C. Maioli, R. Granata, F. Scarlatti. - In: JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. - ISSN 0391-4097. - 28:11(2005 Dec), pp. 1009-1014.
Abstract:
BACKGROUND:
During application of orthodontic force on the tooth, various molecular parameters associated with tissue remodeling are changed. IGF-I is a regulatory protein produced during periodontal regeneration. IGF binding proteins-3 (IGFBP-3), a specific IGF-I binding protein, is the major regulatory factor of IGF-I activity.
OBJECTIVES:
We tested the hypothesis that changes in the IGF-I/ IGFBP-3 system occur during fixed force application to the tooth and that these changes are detectable in the gingival crevicular fluid (GCF).
METHODS:
IGFBP-3 and IGF-I secretion into gingival crevicular fluid (GCF) was analyzed by Western blotting and immunoradiometric assay (IRMA), respectively, in GCF of 6 healthy subjects just prior to and during orthodontics treatment using fixed appliances.
RESULTS:
We observed a significant time-dependent decrease of IGFBP-3 content in GCF during orthodontic treatment (4 h and 10 days). Reduction in levels of intact, glycosylated 47 kDa form of IGFBP-3 was associated with its degradation and the appearance of intermediate breakdown products. IGF-I levels were significantly increased 4 h after application of orthodontic force, while they were significantly reduced 10 days after the start of treatment.
CONCLUSIONS:
IGFBP-3 secretion into GCF and its molecular structure are modified by the fixed force of orthodontic treatment. Alterations in IGFBP-3 appear to be unrelated to the binding to IGF-I, suggesting an IGF-independent role of this binding protein in tooth movement.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
M. Toia, R. Galazzo, C. Maioli, R. Granata, F. Scarlatti
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