The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer
Articolo
Data di Pubblicazione:
2013
Citazione:
The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer / C. Dieci, V. Lodde, F. Franciosi, I. Lagutina, I. Tessaro, S.C. Modina, D.F. Albertini, G. Lazzari, C. Galli, A.M. Luciano. - In: BIOLOGY OF REPRODUCTION. - ISSN 0006-3363. - 89:3(2013 Aug 07). [10.1095/biolreprod.113.110577]
Abstract:
In the pig, the efficiency of in vitro embryo production and somatic cell nuclear transfer (SCNT) procedures remains limited. It has been suggested that prematuration treatments (pre-IVM) based on the prolongation of a patent bidirectional crosstalk between the oocyte and the cumulus cells through gap junction mediate communication (GJC), together with the maintenance of a proper level of cAMP, could improve the developmental capability of oocytes. The aim of this study was to assess: (i) dose dependent effects of cilostamide on nuclear maturation kinetics; (ii) the relationship between treatments on GJC functionality and large-scale chromatin configuration changes; (iii) and the impact of treatments on developmental competence acquisition after parthenogenic activation (PA) and SCNT. Accordingly, COC were collected from 3-6 mm antral follicles and cultured for 24 h in defined culture medium with or without 1 μM cilostamide. GJC functionality was assessed by Lucifer Yellow microinjection, while chromatin configuration was evaluated by fluorescence microscopy after nuclear staining. Cilostamide administration sustained functional coupling up to 24 h of culture and delayed meiotic resumption as only 25.6% of cilostamide-treated oocytes reached ProMI stage compared to the control (69.7%; P<0.05). Moreover, progressive chromatin condensation was delayed before meiotic resumption based upon G2/M biomarker phosphoprotein epitope acquisition using immunolocalization. Importantly, cilostamide treatment under these conditions, improved oocyte developmental competence as reflected in higher blastocyst quality after both parthenogenetic activation and SCNT
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chromatin; Cumulus cells; Cyclic adenosine monophosphate (cAMP); Embryonic development; Gamete biology; Gap junctions; Meiosis; Oocyte; Parthenogenetic activation; PDE3; Phosphodiesterases; Porcine/pig; SCNT
Elenco autori:
C. Dieci, V. Lodde, F. Franciosi, I. Lagutina, I. Tessaro, S.C. Modina, D.F. Albertini, G. Lazzari, C. Galli, A.M. Luciano
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