Data di Pubblicazione:
2013
Citazione:
Suitability of the measurement imprecision of commun urinary biochemical analytes / D. Szőke, F. Braga, C. Valente, M. Panteghini. - In: BIOCHIMICA CLINICA. - ISSN 0393-0564. - 37:suppl.13(2013), pp. T282.S432-T282.S432. (Intervento presentato al convegno EUROMEDLAB tenutosi a Milano nel 2013).
Abstract:
Background: Imprecision monitoring is central in the
performance evaluation of an analytical system. To be suitable
in clinical setting, the measurement imprecision should fulfil
goals derived from biological variation data of the
corresponding analyte, when available. Here we aimed to
evaluate the long-term imprecision in our laboratory of
measurements of 11 urinary analytes measured on the Roche
Cobas 6000 system.
Methods: Data were obtained for evaluated analytes by
drawing up an internal quality control (QC) programme based
on daily measurements of a lyophilized control material
(Assayed Urine Control – Level 3, Randox Laboratories, lot no.
580UC). CVs were calculated along the whole examined
period (February 2012 to September 2012) (n=165) and
compared with corresponding goals for imprecision derived
from biological variability data (analyte concentration in 24 h
urine sample), with the exception of urinary chloride and
glucose for which biological variability data are missing.
Results: Analyte concentration means and CVs (optimum goals
in parentheses) were as follows: albumin, 182 mg/L, 3.0%
(15.3%); calcium, 194 mg/L, 2.9% (6.9%); chloride, 258
mmol/L, 2.2% (not available); creatinine, 1.91 g/L, 2.1% (6.0%);
glucose, 2.78 g/L, 1.9% (not available); magnesium, 323 mg/L,
3.5% (11.4%); phosphate, 852 mg/L, 2.0% (6.6%); potassium,
105 mmol/L, 2.1% (6.8%); sodium, 193 mmol/L, 1.9% (6.0%);
urea, 14.7 g/L, 2.5% (5.7%); and urate, 214 mg/L, 2.7% (6.2%).
Conclusions: Our study shows that in routine laboratory
practice and over a clinically and analytically relevant timespan,
the imprecision of the common urinary analyte
measurements on the Cobas 6000 system fulfils optimum
goals of analytical performance. Although intra-individual
biological variability of urinary analytes is usually relatively high,
resulting in less stringent analytical goals when compared to
those of serum measurements, the excellent CVs of urinary
analyte measurements qualify the suitability for clinical
application of these tests.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
D. Szőke, F. Braga, C. Valente, M. Panteghini
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