Prolactin and proinflammatory cytokine expression at the fetomaternal interface in first trimester miscarriage
Articolo
Data di Pubblicazione:
2013
Citazione:
Prolactin and proinflammatory cytokine expression at the fetomaternal interface in first trimester miscarriage / E. Garzia, R. Clauser, L. Persani, S. Borgato, G. Bulfamante, L. Avagliano, F. Quadrelli, A.M. Marconi. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - 100:1(2013 Jul), pp. 108-115.e2. [10.1016/j.fertnstert.2013.02.053]
Abstract:
OBJECTIVE:
To investigate the expression of prolactin (PRL), PRL-receptor (PRL-R), and the TH1 cytokines interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) at the maternofetal interface.
DESIGN:
Case-control study.
SETTING:
University hospital unit of gynecology and obstetrics and research laboratories.
PATIENT(S):
Women undergoing suction curettage for spontaneous miscarriage (study group) and voluntary termination of pregnancy (control group) in the first trimester.
INTERVENTION(S):
Samples of decidua and villi collected and histologically examined at the time of suction curettage.
MAIN OUTCOME MEASURE(S):
Evaluation of all villous samples for karyotype with only euploid cases included; detection of transcripts of PRL, PRL-R, TNF-α, IFN-γ, and IL-2 by qualitative reverse-transcriptase-polymerase chain reaction (RT-PCR); investigation of pattern and site of expression by immunohistochemistry.
RESULT(S):
In both groups, PRL-R and IFN-γ were broadly expressed. The expression of PRL was impaired or absent in the villi of the study group compared with controls. Expression of TNF-α was reduced, although not statistically significantly, in both decidual and villous samples of the study group. Immunohistochemical analysis showed the lack of IL-2 expression in decidual specimens of the control group versus the full expression shown in the study group.
CONCLUSION(S):
Our results highlight the correspondence between PRL expression and vital pregnancy and the involvement of the TH1 cytokines with different specific roles at the implantation site. Prolactin and IL-2 may reciprocally influence expression.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cytokines; decidua; miscarriage; prolactin; villi
Elenco autori:
E. Garzia, R. Clauser, L. Persani, S. Borgato, G. Bulfamante, L. Avagliano, F. Quadrelli, A.M. Marconi
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