SURFACE CHEMISTRY EVALUATION IN THE DESIGN AND FABRICATION OF MICRO-DEVICES FOR PROTEIN ANALYSIS
Tesi di Dottorato
Data di Pubblicazione:
2013
Citazione:
SURFACE CHEMISTRY EVALUATION IN THE DESIGN AND FABRICATION OF MICRO-DEVICES FOR PROTEIN ANALYSIS / V. Pistis ; docente guida: E. Santaniello ; tutor: M. Chiari ; coordinatore : F. Bonomi. UNIVERSITA' DEGLI STUDI DI MILANO, 2013 Feb 19. 25. ciclo, Anno Accademico 2012. [10.13130/pistis-valentina_phd2013-02-19].
Abstract:
In this Ph.D. thesis, the problem of the coating and adhesion of polymers onto selected surfaces
has been investigated with the aim of designing micro-devices and/or microarrays for protein
analysis. In the first part of the thesis, the micro-fabrication of a device for isoelectric focusing has
been studied. The development of this device has required the covalent attachment of hydrogel
plugs functionalized with pH buffering monomers on an Indium Tin Oxide (ITO) flat surface. The
two ITO-coated slides are separated by silicon frames (both 3mm thick) stuck on the surface and
the whole device was named as Micro Parallel Isoelectric focusing Device (MPID). The problem of
the adhesion of the hydrogel plugs onto the ITO anode under the high vacuum conditions of the
MALDI-TOF/TOF analysis has been solved, by properly selecting the percentage of monomers and
related polymerization conditions.
The MPID fabricated in accord to previous observations did not afford a completely efficient
isolectric separation of the model protein mixture (commercial carbonic anhydrase). Furthermore,
the proteins were absorbed onto plugs too tightly for a satisfactory, direct analysis by MALDITOF/
TOF of the plugs.
At this stage of the Ph.D. program, the MPID project has been abandoned and the second part of the
Ph.D. thesis has been directed towards the study of a functional coating onto different surfaces
using a polymer that is the result of the co-polymerization of N,N-dimethylacrylamide (DMA), 3-
(trimethoxysilyl)-propylmethacrylate (MAPS) and acryloyloxysuccinimide (NAS) [ Copoly (DMANAS-
MAPS)]. It has been shown that this copolymer is able to generate thin layer of nanometer
size onto inorganic substrates like glass, silicon oxide, or gold, typically used for the development
of microarrays or bio-sensors for immunoassays. Applications of silicon and gold surfaces coated
by Copoly (DMA-NAS-MAPS) are presented and discussed.
Tipologia IRIS:
Tesi di dottorato
Elenco autori:
V. Pistis
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