Data di Pubblicazione:
2013
Citazione:
CANINE EPIDIDYMAL SPERMATOZOA: CHARACTERISTICS, COLLECTION AND CRYOPRESERVATION / S. Varesi ; tutor: G.C Luvoni ; coordinator: F. Cremonesi. UNIVERSITA' DEGLI STUDI DI MILANO, 2013 Mar 26. 25. ciclo, Anno Accademico 2012. [10.13130/varesi-sara_phd2013-03-26].
Abstract:
Epididymal spermatozoa represent the only source of genetic material when the
male dies unexpectedly or undergoes orchiectomy for medical reasons. Yet, in
individuals of high genetic or emotional value that cannot mate or ejaculate semen, the
collection of spermatozoa from in situ epididymides might be an option to obtain
progeny.
The aims of the studies presented here were to describe the characteristics of
spermatozoa collected from the different regions of the epididymis (caput, corpus, and
cauda) and to investigate the feasibility of percutaneous epididymal sperm aspiration
(PESA) in dogs. Furthermore, the preservation of DNA integrity in canine epididymal
frozen spermatozoa and the potential protective effect of the antioxidant melatonin on
post-thaw sperm quality were evaluated.
Overall results showed that canine epididymal spermatozoa undergo several
modifications in the epididymis, leading to mature cells which are stored in the
epididymal cauda. From this site, spermatozoa can be retrieved by PESA and the quality
of the sperm population is similar to that of spermatozoa collected in vitro, although a
wide variation amongst animals was observed.
The cryopreservation of epididymal spermatozoa showed that the DNA integrity
is well preserved, but a protective effect of melatonin on post-thaw sperm quality has
not been demonstrated.
The DNA stability after thawing is particularly relevant for epididymal
spermatozoa which potential use in assisted reproductive techniques is mainly after
storage. However, other sperm characteristics as motility and acrosomal integrity are
compromised by freezing and further investigations should be focused on their
preservation.
Tipologia IRIS:
Tesi di dottorato
Keywords:
canine; epididymal; spermatozoa; cryopreservation
Elenco autori:
S. Varesi
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