Data di Pubblicazione:
2013
Citazione:
Presence of endogenous prednisolone in human urine / M. Fidani, M.C. Gamberini, G. Pompa, F. Mungiguerra, A. Casati, F. Arioli. - In: STEROIDS. - ISSN 0039-128X. - 78:2(2013 Feb), pp. 121-126. [10.1016/j.steroids.2012.10.020]
Abstract:
The possibility of an endogenous presence of the glucocorticoid prednisolone has already been demonstrated
in bovine and horse urine, with the aim of clarifying its origin in this matrix, which is used by
official agencies for the control of illicit treatments. From this point of view, the endogenous nature of
prednisolone could be a major topic in doping control of both amateur and professional human athletes.
A study was therefore made on 34 human volunteers (13 males and 21 females; aged 22–62) to detect
the presence of prednisolone in their urine by HPLC–MS3. One of the volunteers underwent vernal allergy
treatment with betamethasone for two subsequent years. An investigation was carried out with the aim
of verifying if the suppression, and the circadian rhythm, of cortisol urinary levels could also apply to
prednisolone. The results of the study show that prednisolone was present in the urine of all 34 volunteers,
with a concentration very close to 100-times lower that of cortisol, with no dependence on gender.
The same ratio (1/100) was observed in the prednisolone and cortisol levels detected during the 24 h
together with the suppression of prednisolone by betamethasone treatment.
These data demonstrate the endogenous nature of low concentrations of prednisolone in human urine,
and motivate further studies about the biosynthetic pathways of this corticosteroid and its relationship
with stress in humans, as already described in cows.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cortisol; Endogenous; Human urine; Prednisolone
Elenco autori:
M. Fidani, M.C. Gamberini, G. Pompa, F. Mungiguerra, A. Casati, F. Arioli
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