Chemical composition of lipids present in cat and dog oocyte by Matrix-Assisted Desorption Ionization Mass Spectrometry (MALDI- MS)
Articolo
Data di Pubblicazione:
2012
Citazione:
Chemical composition of lipids present in cat and dog oocyte by Matrix-Assisted
Desorption Ionization Mass Spectrometry (MALDI- MS) / M. Apparicio, C.R. Ferreira, A. Tata, V.G. Santos, A.E. Alves, G.Q. Mostachio, E.A. Pires-Butler, T.F. Motheo, L.C. Padilha,
E.J. Pilau, F.C. Gozzo, M.N. Eberlin, E.G. Lo Turco, G.C. Luvoni, W.R.R. Vicente. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 47:Suppl. 6(2012 Dec), pp. 113-117. ((Intervento presentato al 7. convegno Quadrennial International symposium on canine and feline reproduction tenutosi a Whistler, Canada nel 2012 [10.1111/rda.12003].
Abstract:
The aim of the present study was to investigate the level of
information on the chemical structures and relative abundances
of lipids present in cat and dog oocytes by matrixassisted
laser desorption mass spectrometry (MALDI-MS).
The MALDI-MS approach requires a simple analysis workflow
(no lipid extraction) and few samples (two or three
oocytes per analysis in this work) providing concomitant
profiles of both intact phospholipids such as sphingomyelins
(SM) and phosphatidylcholines (PC) as well as triacylglycerols
(TAG). The lipids were detected in oocytes by MALDI using
dihydroxybenzoic acid (DHB) as the matrix. The most
abundant lipid present in the MS profiles of bitch and queen
oocytes was a PC containing 34 carbons and one unsaturation
[PC (34:1)]. Oocytes of these two species are characterized by
differences in PC and TAG profiles detected qualitatively as
well as by means of principal component analysis (PCA). Cat
oocytes were mainly discriminated by more intense C52 and
C54 TAG species and a higher number of unsaturations,
indicating predominantly linoleic and oleic fatty acyl residues.
Comparison of the lipid profile of bitch and queen oocytes
with that of bovine oocytes revealed some similarities and also
some species specificity: TAG species present in bovine oocytes
were also present in bitches and queens; however, a more
pronounced contribution of palmitic, stearic and oleic fatty
acid residues was noticed in the lipid profile of bovine oocytes.
MALDI-MS provides novel information on chemical lipid
composition in canine and feline oocytes, offering a suitable
tool to concomitantly monitor, in a nearly direct and simple
fashion the composition of phospholipids and TAG. This
detailed information is highly needed to the development of
improved protocols for in vitro culture and cryopreservation of
cat and dog oocytes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cat ; dog ; oocytes ; lipids ; MALDI- MS
Elenco autori:
M. Apparicio, C.R. Ferreira, A. Tata, V.G. Santos, A.E. Alves, G.Q. Mostachio, E.A. Pires Butler, T.F. Motheo, L.C. Padilha, E.J. Pilau, F.C. Gozzo, M.N. Eberlin, E.G. Lo Turco, G.C. Luvoni, W.R.R. Vicente
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