In vitro Survival of Follicles Collected from Domestic Cats’ Ovaries at Different Stages of Oestrous Cycle and Cultured with IGF-1
Articolo
Data di Pubblicazione:
2012
Citazione:
In vitro Survival of Follicles Collected from Domestic Cats’ Ovaries at Different
Stages of Oestrous Cycle and Cultured with IGF-1 / E.A. Alves, L. Padilha, P.A. Savi, M.F. Apparicio, G.Q. Mostachio, T.F. Motheo, E.A. Pires-Buttler, W.R.R. Vicente, G.C. Luvoni. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 47:Suppl. 6(2012 Dec), pp. 109-112. ((Intervento presentato al 7. convegno Quadrennial International symposium on canine and feline reproduction tenutosi a Whistler, Canada nel 2012 [10.1111/rda.12001].
Abstract:
Optimal conditions for in vitro culture of feline ovarian
follicles have not yet been defined. Follicular development is
regulated by intraovarian growth factors, as insulin-like
growth factor (IGF-1), and during the different stages of the
oestrous cycle, follicles are exposed to specific hormonal
environments. The aim of this study was to investigate the
effect of IGF-1 on in vitro growth and granulosa cell (GC)
viability of preantral follicles collected from domestic cats at
follicular and luteal phases of the oestrous cycle. Oestrus and
ovulation were induced in 12 cats. A total of 39 and 32 follicles
collected at the follicular and luteal phases, respectively, were
individually cultured in vitro for 6 days in minimum essential
medium media supplemented with or without IGF-1 (100 ng/
ml). Follicles collected during the follicular phase and cultured
without IGF-1 displayed a significant increase in size and
higher GC viability (46.5 ± 22.1 lm, 66.7%, respectively) than
that of follicles collected at the luteal phase and cultured
without IGF-1 (26.7 ± 14.4 lm, 50%, respectively; p < 0.05).
In contrast, when IGF-1 was added to the culture medium, no
differences were observed in size or GC viability between
follicles collected at the two phases of the cycle. Nonetheless,
follicles collected at the luteal phase and cultured with IGF-1
had a significant increase in their diameter and GC viability
(31.9 ± 15.9 lm, 63.6%, respectively) than that cultured
without IGF-1 (26.7 ± 14.4 lm, 50%, respectively; p < 0.05).
These data suggest that in vitro growth and GC survival of
feline preantral follicles are affected by the oestrous cycle
phase, and the IGF-1 exerts a positive effect on follicles
collected at the luteal phase.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cat ; ovarian follicle ; in vitro culture
Elenco autori:
E.A. Alves, L. Padilha, P.A. Savi, M.F. Apparicio, G.Q. Mostachio, T.F. Motheo, E.A. Pires Buttler, W.R.R. Vicente, G.C. Luvoni
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