Data di Pubblicazione:
2012
Citazione:
Cryosurvival of ex situ and in situ feline oocytes / G.C. Luvoni. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 47:Suppl. 6(2012), pp. 266-268. ((Intervento presentato al 7. convegno Quadrennial International symposium on canine and feline reproduction tenutosi a Whistler, Canada nel 2012 [10.1111/rda.12040].
Abstract:
Cryosurvival of feline oocytes preserved as isolated cells (ex situ)
or enclosed in ovarian follicles (in situ) has been demonstrated,
and significant advances have recently been achieved. However,
an ideal protocol for oocyte cryopreservation has not been
established to date because of extreme sensitivity of the
structural complex to chilling injury. Several factors, such as
stage of maturation, membrane permeability and plasticity of
the cytoskeleton, affect cryosurvival of the oocyte. Also,
intercellular communications between cumulus cells and oocyte
are compromised after freezing or vitrification of ex situ or
in situ cumulus–oocyte complexes, which has a detrimental
effect on oocyte maturational competence. Despite these issues,
embryo development, pregnancies and live kittens have been
obtained after in vitro fertilization (by ICSI) and transfer of
embryos derived from cryopreserved oocytes. It is a general
belief that the efficiency of cryopreservation would increase
through a better understanding of oocyte responses to cryoprotectants,
cooling rates and all the physical events occurring
during the exposure of feline oocytes to low temperatures.
Cryobanking of feline oocytes would significantly contribute to
the preservation of rare genotypes and to the maintenance of a
valuable source of genetic material for research applications.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cat ; cryopresrevation ; oocytes
Elenco autori:
G.C. Luvoni
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